TABLE 3.

Equilibrium dissociation constants of the complexes between the hPRL variants and PRLR-ECD (rat or human)

	Kd
	Site 1		Sites 2+3
	nm
hPRL variants	Rat ECD	Human ECD	Rat ECD	Human ECD
WT	10.2 ± 1.5	6.5 ± 1.0	11,000 ± 700	32,800 ± 1,800
T14P	12.6 ± 1.3	ND	26,300 ± 9,600	NDa
Nter	8.3 ± 0.4	5.9 ± 0.1b	4,900 ± 1,000	32,800 ± 14,700
PGGA	6.2 ± 1.4	ND	3,700 ± 1,900	ND
PGGA-T14P	3.5 ± 0.2	ND	373 ± 72	ND
Full-oPL	1.2 ± 0.1	2.1 ± 0.6	312 ± 36	16,400 ± 2,800

^a ND, not determined.

^b From Ref. 20.