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. 2010 Jan 8;285(11):8454–8462. doi: 10.1074/jbc.M109.075036

FIGURE 2.

FIGURE 2.

MTMR4 functions downstream of TβRI. A, MTMR4 inhibits constitutively active TβRI (GGD)-induced CAGA-luciferase reporter transcriptional activation in a dose-dependent manner. L17 cells were cotransfected with CAGA reporter, pCMV-hRL, pCS2-GGD-HA and increasing amounts of pYFP-MTMR4. Luciferase assays were performed as in Fig. 1C. B, MTMR4 inhibits Smad3-induced CAGA-luciferase reporter transcriptional activation in a dose-dependent manner. L17 cells were co-transfected with CAGA reporter, pCMV-hRL, pCMV-Myc-Smad3, and increasing amounts of pYFP-MTMR4. Luciferase assays were performed as in Fig. 1C. C, MTMR4 fails to inhibit constitutively active Smad3 (SD)-induced CAGA-luciferase reporter transcriptional activation. L17 cells were co-transfected with CAGA reporter, pCMV-hRL, pRK5-HA-Smad3 (SD) and increasing amounts of pYFP-MTMR4. Luciferase assays were performed as in Fig. 1C.