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. 2010 Jan 11;285(11):8472–8480. doi: 10.1074/jbc.M109.069450

FIGURE 4.

FIGURE 4.

A, mRNA microarray analysis of PEG3 mRNA in 32 surgical GBM specimens and five surgical non-tumor brain specimens (NTB) showing decreased PEG3 mRNA expression in GBM (p < 0. 001, t test). B, real-time PCR of PEG3 mRNA expression in five human non-tumor brain specimens and 10 human GBM specimens. Samples were assayed in triplicate. Data shown are means ± S.E. C, mRNA microarray analysis for PEG3 in low-grade oligodendroglioma (LGO; n = 15), low-grade astrocytoma (LGA; n = 15), anaplastic astrocytoma (AA; n = 7), and GBM (n = 32). p < 0.04 for GBM versus low-grade astrocytoma and low-grade oligodendroglioma. D, methylation-specific PCR for methylated (M) and unmethylated (U) PEG3 alleles in primary human non-tumor brain, low-grade astrocytoma, and GBM specimens (upper panels) or in five cultured glioma cell lines (lower panel). E, real-time PCR showing the effect of methyltransferase inhibition using 5-azadeoxycytidine (5-aza-dC) on PEG3 mRNA expression in human U87 glioma cells. F, array comparative genomic hybridization analysis of the PEG3 locus on chromosome 19q13.34 in 17 surgical GBM specimens. DNA copy number is plotted in the upper panel. The genomic location of the PEG3 gene (arrow) is shown in the lower panel.