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. 1997 Dec 9;94(25):13475–13480. doi: 10.1073/pnas.94.25.13475

Figure 2.

Figure 2

Affinity cleavage (18, 19) of the AdMLP TATA box by yTBP derivatives. (A) Autoradiogram of a 12% high resolution sequencing gel analyzing the products of affinity cleavage of the coding strand of an 80-bp fragment of the AdMLP. The diagram on the left shows the sequence of the AdMLP TATA box; “upstream” and “downstream” refer to the location of the DNA relative to the TATA box. The left-most lane shows a DNA control. Histograms illustrating cleavage by E188C-OP and K97C-OP at each base pair in the AdMLP (B) and CYC1 (C) promoters. The extent of cleavage at each position (above a cupric ion, hydrogen peroxide, and mercaptopropionic acid control) is proportional to the length of the arrow. The TATA box is shaded gray.