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Coomassie-stained SDS–PAGE analysis of recombinant CPCuda. Lane 1, molecular-weight markers; lane 2, total cell lysate before induction; lane 3, total cell lysate after induction; lane 4, eluate from Q-Sepharose at 500 mM NaCl; lane 5, pooled fractions after gel filtration (Superdex 200).
