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RPS14 gene expression and generation of enucleated RBC in a LDA. On day 8, 5q (del) clones were pooled [>99% 5q (del) clones as determined by FISH analysis]. RPS14 gene expression was measured by real time RT-PCR and normalized to β2 microglobulin: ΔCt=CtRPS14 − Ctβ2m; the higher the ΔCt, the lower the gene expression. We then determined the percentage of enucleated RBC on day 18. A similar analysis was performed with normal clones.
