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. 2009 Oct 8;95(3):456–463. doi: 10.3324/haematol.2009.006395

Figure 4.

Figure 4.

Thalidomide (Thd) inhibits phosphorylation of Src and ERK kinases in response to fibronectin (FN). (A) Western blot detection of Src phosphorylated at Y416 and at Y527 in Raji cells exposed or not to fibronectin (10 μg/mL) and incubated with the indicated concentrations of thalidomide. The membrane was stripped and reprobed with anti p60 Src and with anti β-actin antibody. (B) Western blot detection of phosphorylated p42 and p44 ERK in Raji cells cultured in the same conditions as in A. The blot was subsequently stripped and re-probed with anti-ERK antibody. Similar results were obtained from IM9 cells.