Fig. 1.

Immunohistological analysis of B cell zones in tumor-draining lymph nodes from breast cancer patients. a Immunohistochemistry staining for CD20, CD23 and Ki67 in serial sections of a tumor-draining lymph node (bar = 1,000 μm). b Merged image of the three stains in the zone highlighted in a. Masks of different colors were applied to each marker: CD20 yellow, CD23 blue, and Ki67 pink (bar = 1,000 μm). c Number of zones positive for CD20, CD20 and CD23 or a combination of the three markers in each lymph node (average and standard deviations are shown, n = 32)