Figure 4.

Localization of GADD45A, IGFBP5 and TSP2 mRNA in healthy and atretic follicles. These genes were expressed more in F2 than in F1 in QPCR analysis. (A, C, E, G, I and K) DIG-labeled anti-sense cRNA probes were used. (B, D, F, H, J and L) DIG-labeled sense cRNA probes were used. Seven-micrometer sections of bovine follicles were hybridized with each probe. GADD45A (A, B, C and D) and TSP2 (I, J, K and L) mRNA were found in both granulosa cells (GC) and theca layer (TL) of atretic follicle, whereas it was localized in only GC of healthy follicle. IGFBP5 mRNA (E, F, G and H) was localized in GC and TL of atretic follicle but not found in healthy follicle. Scale bar = 20 μm.