FIG. 2.

(A) Thyroid cells express estrogen receptor (ER). Nuclear (NE) and cytoplasmic (CE) protein fractions (15 μg) were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, and Western blot analysis, for ER-α (45 KDa) and ER-β (60 KDa), was performed. MCF-7, ER-positive cells were used as positive control. (B) ER is complexed with Akt and HSP90 in thyroid cells. Cell lysates were subjected to immunoprecipitation with antibodies to human ER-α and ER-β followed by Western blot analysis for ER-α, ER-β, HSP90, and Akt, respectively (top to bottom panel of immunoprecipitation and Western blot). MCF-7 (ER positive) was used as a positive control for detection of Hsp90 and Akt complex with ER.