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. 2010 Mar 8;5(3):e9585. doi: 10.1371/journal.pone.0009585

Figure 1. Characterization of E. coli LPS-induced BMDC maturation.

Figure 1

(A) Purity of BMDCs. Bone marrow cells were cultured in the presence of GM-CSF and IL-4 for 7 days and analyzed by flow cytometry for the presence of CD11c and MHC II. (B) Induction of CD40 and CD80 by LPS. BMDCs were LPS-stimulated for 24 hours and analyzed for the induction of CD40 and CD80 by flow cytometry. Error bars correspond to standard deviation from 5 independent experiments. (C) Cytokine induction. BMDCs were LPS-stimulated for the indicated periods of time and cytokines were assayed from culture supernatant by ELISA. The presented data correspond to a representative experiment.