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. 2010 Mar 8;5(3):e9585. doi: 10.1371/journal.pone.0009585

Figure 3. Involvement of the NF-κB pathway in JunB induction in BMDCs.

Figure 3

(A) Inhibition of p65/RelA nuclear translocation by BAY11-7085. BMDCs were left unstimulated or were stimulated by LPS in the presence of either BAY 11-7085 or solvent (DMSO) for 1 hour. After cell fixation, nuclei were stained with Hoescht 33342 and p65/RelA was detected by indirect immunofluorescence. (B) Inhibition of JunB induction by BAY11-7085. BMDCs were stimulated by LPS in the presence of either BAY 11-7085 or solvent (DMSO) for various periods of time. The levels of IκBα and JunB were assayed by immunoblotting, taking GAPDH as an invariant electrophoresis loading control. The experiments were reproduced 3 times.