Skip to main content
. 2010 Mar 8;5(3):e9587. doi: 10.1371/journal.pone.0009587

Figure 2. Targeting strategy for the mouse Sp2 gene.

Figure 2

A) Schematic representation of the Sp2 protein structure, the wild type Sp2 allele, the targeting vector and the targeted Sp2lzn allele. The Sp-box (Sp), two glutamine-rich domains (Q-rich), the btd-box (btd) and the three zinc fingers (ZnF) of the Sp2 protein are indicated. Connecting lines with the corresponding murine Sp2 gene region indicate the derivation of N-terminal parts of the Sp2 protein. The Sp-box and two glutamine-rich regions are encoded by exon 3. In the targeted Sp2 locus exon 3 and the IRES-lacZ-neo cassette are floxed (black arrowheads). White arrowheads depict PCR primer locations (P1, P2 and P3). S, SacI sites. B) Southern blot analysis of transfected ES cells for the Sp2wt allele (6.9 kb) and the targeted Sp2lzn allele (3.2 kb) using SacI-restricted genomic DNA and the 387 bp probe indicated in Figure 2A. C) PCR analysis of E13.5 embryos for the Sp2lzn allele with the indicated primer combination (P1, P2 and P3).