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. 2010 Mar 8;5(3):e9587. doi: 10.1371/journal.pone.0009587

Figure 5. Generation of Sp2null mice.

Figure 5

(A) Mice carrying an Sp2null allele were obtained by crossing heterozygous Sp2wt/lzn mice with CAG-Cre mice [18] thereby removing the selection marker (IRES-LacZ-Neo) and exon 3. Black arrowheads depict loxP sites, white arrowheads indicate PCR primer locations. B) PCR analysis of the Sp2 wild type (wt) and the knockout (ko) allele using primers P1, P2 and P4 indicated in panel A. C) Immunoblot analysis of Sp2 in extracts of primary mouse embryonic fibroblasts derived from E9.5 embryos with the indicated genotype. The asterisk denotes a cross-reacting protein.