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. 2010 Mar 8;5(3):e9587. doi: 10.1371/journal.pone.0009587

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Baur et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) An Sp2null embryo at E9.5 in comparison to wild type (Sp2wt) and heterozygous (Sp2+/−) littermates. B) An Sp2null embryo at E9.5 in direct comparison to an E8.5 wild type embryo (Sp2wt). C) Serial longitudinal sections of heterozygous Sp2 (Sp2+/−) and Sp2null embryos from day E9.0. Sp2null embryos are smaller, have not turned and have an open cranial neural tube (Nt). Ne, neural epithelium. D) Whole-mount in situ hybridization on E7.5 embryos using the mesodermal marker Brachyury as a probe.