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A) Crossing of heterozygous Sp2wt/lzn mice with MeuCre40 mice resulted in generation of an Sp2cko allele in which the lacZ-Neo fusion gene is absent. B) PCR analysis of the Sp2 wild type (wt), Sp2-IRES-lacZ-Neo (lzn) and Sp2cko (cko) alleles with the indicated primers. C) Western blot analysis of Sp2 in extracts of Sp2cko/cko, Sp2cko/wt and Sp2wt/wt MEFs.
