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. 2010 Mar 8;5(3):e9588. doi: 10.1371/journal.pone.0009588

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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In A, HEK 293 cells were transfected with plasmids for the expression of HA-hTTP (red) and Flag-CIN85 (Green), or (B) HA-MEKK4 (red) and Flag-CIN85 (green). The cells were stained with primary antibodies, either an anti-HA polyclonal or anti-Flag monoclonal, followed by the secondary antibodies Alexa 594 anti-rabbit (red) or Alexa 488 anti-mouse (green), respectively. Nuclei (blue) were stained with DAPI. The cells were visualized and images obtained by confocal microscopy. The merged images of two protein signals, indicating areas of apparent co-localization, are shown in yellow.