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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Cancer Prev Res (Phila). 2010 Feb 9;3(3):331–338. doi: 10.1158/1940-6207.CAPR-09-0076

Figure 2.

Figure 2

Effects of curcumin on components of the translational machinery. (a) NOM9, NOM9-CT, MSK-Leuk1s, SCC4 and UMSCC22B cells were treated with DMSO or various doses of curcumin for 30 min. Whole cell lysates were then prepared and Western blotting was performed using antibodies against the proteins (total protein and phosphorylated forms identified with the letter “p” listed to the left of each row. (b) NOM-9CT cells were pretreated with 5 μM MG132 for 24 hrs before treating with 50 μM curcumin for 30 min. The cell lysates were subjected to western blot analysis on 12% PAGE in the presence of SDS using antibodies against 4E-BP1, eIF4E and cyclin D1. The data are from one experiment, which was repeated with similar results.