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. 2010 Feb 9;102(5):844–851. doi: 10.1038/sj.bjc.6605561

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Copyright © 2010 Cancer Research UK

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Expression of TGF-β signalling and inhibitory effect of Ki26894. (A) Expression level of TGF-β receptor and phospho-Smad2. The overexpression of TGF-β receptor type I (TβR-I), type II (TβR-II), and phospho-Smad2 (P-Smad2) was observed in scirrhous gastric cancer cell lines, OCUM-2MLN and OCUM-12, but not in non-scirrhous gastric cancer cell lines, MKN-45 and MKN-74. (B, C) Effects of Ki26894 on TGF-β-induced Smad2 phosphorylation of gastric cancer cells. PANC-1 was used as positive control of Smad2 phosphorylation. Transforming growth factor-β stimulated Smad2 phosphorylation in PANC-1, OCUM-2MLN, and OCUM-12 cells. Total Smad2/3 expression was recognised in all four gastric cancer cell lines and no difference in expression level was found on addition of TGF-β1 or Ki26894. Transforming growth factor-β1 (10 ng ml⁻¹) stimulated Smad2 phosphorylation in both scirrhous gastric cancer cell lines, but not in non-scirrhous cancer cell lines, MKN-45 and MKN-74. Smad2 phosphorylation was decreased in a dose-dependent manner by Ki26894 in scirrhous gastric cancer cell lines. PANC-1, a pancreas cancer cell line, was used as positive control of Smad2.