Figure 2.

miR-145- and miR-133a-binding sites in FSCN1 3′-UTR. (A) A luciferase assay using the vector encoding full length of FSCN1 3′-UTR (position 51–1180). BOY cells were transfected with 5 ng vector and 10 nM microRNAs. The Renilla luciferase values were normalised by firefly-luciferase values. (B) Luciferase assays using the vectors encoding putative conserved target sites of FSCN1 3′-UTR identified with the TargetScan database: four conserved sites for miR-145 and one site for miR-133a. (C) Luciferase assays using the mutated vectors in which the specific sites targeted by the microRNAs were deleted.