Figure 2.

Effects of interferon alpha (IFN-α) treatment on NEDD8 ultimate buster 1 (NUB1) mRNA expression levels in renal cell carcinoma (RCC) cell lines. (A) Nine RCC cell lines were treated with or without 1000 IU ml^–1 of IFN-α for 6 h. The NUB1 expression levels of all samples were then determined using Taqman real-time PCR. Three individual experiments were performed. Values are means±s.d. of the gene expression level relative to that in the control, given an arbitrary value of 1. ^*P <0.05 (Student’s t-test, n=3). (B) IFN-α-sensitive (ACTHN, OCUU3) and -resistant (A498, caki-1) RCC cell lines were incubated for 19 h with various concentrations of IFN-α as indicated. NUB1 expression levels were determined by the same method as above.