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. 2010 Feb 16;102(5):873–882. doi: 10.1038/sj.bjc.6605574

Figure 6.

Figure 6

Knockdown of NEDD8 ultimate buster 1 (NUB1) expression promotes cell growth and suppresses interferon alpha (IFN-α)-induced inhibition of cell proliferation . (A, a) A498 cells transfected with negative control short interfering RNA (siRNA) (left) or NUB1 siRNA (right) were harvested 72 h after transfection and total cell lysates were analysed by western blotting using an anti-NUB1 antibody to evaluate the efficacy of NUB1 siRNA. (B, a) IFN-α-untreated 4THUR cells transfected with negative control (left), 4THUR cells, which were simultaneously treated with 1000 IU ml–1 IFN-α for 72 h, transfected with negative control (middle) or NUB1 siRNA (right) were followed up by the aforementioned analysis. (A, b; B, b) Viable cells were counted and the numbers were normalised on the basis of negative control cells. Three individual experiments were performed and the means±s.d. are presented; *P<0.05 compared with the control (Student's t-test, n=3).