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. Author manuscript; available in PMC: 2011 Feb 24.
Published in final edited form as: J Am Chem Soc. 2010 Feb 24;132(7):2359–2369. doi: 10.1021/ja909451a

Figure 4.

Figure 4

HPLC experiments demonstrating that a constant external source of reducing equivalents is not required for the dehydrogenation of TDP-d-quinovose (18) to TDP-3-keto-6-deoxy-d-glucose (19) by DesII. (A) Incubation of reduced DesII with 18 at 5 and 85 min demonstrating 26% and 55% reaction in terms of 18, respectively (SAM is substoichiometric versus 18 in this experiment). (B) Incubation of nonreduced DesII with 18 in the presence of deproteinized reduced DesII filtrate at 5 and 85 min demonstrating less than 1% reaction at each time point. Peak 14 corresponds to 5'-deoxyadenosine generated as SAM is consumed. Peaks a and b correspond to contaminants in the commercial SAM. See text for details and Figure 3 for a comparison with the deamination reaction.