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pH Dependence for CPS Dyad Mutantsa
| kcat | kcat/Km | |||
|---|---|---|---|---|
| CPS | pKa | pKb | pKa | pKb |
| WTb | 7.4 ± 0.1 | 9.7 ± 0.1 | 7.8 ± 0.1 | 10.0 ± 0.1 |
| Y345F | 7.67 ± 0.01 | 9.65 ± 0.01 | 7.6 ± 0.1 | 9.5 ± 0.1 |
| Y345A | – | – | – | – |
| E380D | 7.3 ± 0.1 | 9.8 ± 0.1 | 7.63 ± 0.01 | 9.43 ± 0.01 |
| E380Q | 9.46 ± 0.01 | 8.75 ± 0.05 | ||
| E380A | – | – | – | – |
(–) indicates no transition observed in the pH–rate profile. WT represents wild-type CPS.
Values previously reported in ref 12.
