. Author manuscript; available in PMC: 2010 Jun 9.

Published in final edited form as: Biochemistry. 2009 Jun 9;48(22):4959–4971. doi: 10.1021/bi900432n

Table 3.

pH Dependence for β-LS Dyad Mutants^a

	k_cat	k_cat/K_m

β-LS	pK_a	pK_a	pK_b
WT^b	8.07 ± 0.05	9.15 ± 0.06	7.95 ± 0.08
Y348F	8.37 ± 0.03	7.25 ± 0.05
Y348A	–	–	–
E382D	8.27 ± 0.08	9.5 ± 0.1	8.3 ± 0.2

(–) indicates no transition observed in the pH–rate profile from no detectable activity below pH 8.3. WT represents wild-type β-LS. Italic type shows the pK values that were further calculated from the observed pH–(k_cat/K_m) and reflects reverse protonation of the protein.

pK_a in the wild-type pH–k_cat profile previously reported in ref 11.