Effectiveness of the sucrose block during electrical stimulation (black bars under traces). A, Schematic diagram of the two-ganglion (M3–M4) preparation. To block neural signals in the connectives, saline in the bow-tie-shaped well was replaced with an isotonic sucrose solution. Reintroduction of saline in the well restored neural transmission. Connectives anterior to M3 were stimulated for 1 s using a suction electrode. Extracellular unit activity was recorded in the connectives posterior to M4. B, Baseline connective recording before stimulation. C, Unit activity in response to electrical stimulation before the block (Pre). D, Recording obtained during the block (blocked). E, Return of electrically induced activity after removal of the block (Post).