Fig. 2.

Folding and unfolding kinetics of the Im7 solvation variants. Chevron plots are shown in the left-hand panels (a, c, e and g); the corresponding initial and endpoint fluorescence data are shown in the right-hand panels (b, d, f and h). Variants created (a) in the N-terminal region and throughout helix I, (c) in helices II and III, and (e) in helix IV. Variants for which ΔΔG°_UN was too small to calculate Φ-values are shown in (g). To facilitate comparison, the fit to the wild-type Im7 data is shown as a black dotted line in all plots. All data were acquired at pH 7.0, 10 °C, in the presence of 0.4 M Na₂SO₄, and fitted to a three-state on-pathway model (see Materials and Methods). The resulting kinetic and thermodynamic parameters are shown in Table 1.