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(a) Fluorescence emission spectra of IV22T (red) and wild-type Im7 (black) in 0 M (continuous line) or 8 M urea (dotted line). (b) Equilibrium denaturation curve of IV22T followed by tryptophan fluorescence. The data are fitted to a two-state transition. 1D 1H NMR spectra of (c) IV22T and (d) wild-type Im7. All data were acquired at pH 7.0, 10 °C, in the presence of 0.4 M Na2SO4.
