Fig. 6.
Fluorescence emission spectra of the overpacked Im7 variants in 0 M (continuous line) or 8 M (dotted line) urea. (a) Overpacked variants with fluorescence properties resembling that of the native wild-type protein; (b) variants that differ slightly in their fluorescence intensity compared with native wild-type Im7; (c) variants that have a fluorescence emission spectrum in 0 M urea that resembles that of the intermediate species (highly fluorescent, λmax ∼ 335 nm).27 (d) Fluorescence emission spectra of Im9 and the overpacked Im9 variants created in this study.