Table 2.

Equilibrium stabilities and m values determined from equilibrium denaturation experiments monitored using far-UV CD (data shown in SI Figure 4)

Variant	Position	ΔG°UN (kJ mol− 1)	MUN (kJ mol− 1 M− 1)
V42F	Helix II	− 19.2 ± 0.8	4.2 ± 0.2
I44F	Helix II	− 16.2 ± 1.9	4.4 ± 0.5
L53F	Helix III	− 13.5 ± 1.2	3.5 ± 0.3
I68F	Helix IV	− 9.3 ± 0.9	3.4 ± 0.2
I72F	Helix IV	− 9.2 ± 0.8	3.2 ± 0.2