Figure 5.

Rh1-shi^ts1 photoreceptors show decelerated voltage responses to light. Mean normalized ERGs (n = 6) to 700 ms stimulus, taken from Figures 1 and 3 after dark adaptation (A) and after light adaptation (B). Wild type is plotted for young (gray) and older (black) flies. white Rh1-shi^ts1 (orange) and Rh1-shi^ts1 (red) at 3 and 6 d posteclosion, respectively, have been plotted together. Similarly, 6 and 9 d posteclosion are shown together. Wild-type (black) and homozygous transgenic controls; Rh1-Gal4 (pink), UAS-shi^ts1 (dark blue) and white UAS-shi^ts1 (blue-green) have been plotted together (bottom). Arrows indicate differences in response waveforms between overexpressed UAS-shi^ts1 and wild type. Age refers to days posteclosion. C, Intracellular recordings of 3 d posteclosion wild-type (black) and Rh1-shi^ts1 (red) photoreceptors. Mean ± SEM shown in traces (n = 9). When Rh1-shi^ts1 displayed the same light response amplitude (red dotted line) as wild type, the dynamics were as slow as the rest of the cells. The histamine mutant hdc^JK910 (green) does not show such delay in its response. D, Representative intracellular recordings of wild type (black) and Rh1-shi^ts1 (red) for a 700 ms light stimulus.