Overexpression of UAS-shits1 changes retinal morphology at 19°C. EM cross-sections of 3 and 9 d posteclosion wild-type (A) and Rh1-shits1 (B) retinas. Scale = 2 μm. C, MVBs present in Rh1-shits1 photoreceptor. Scale = 1 μm. D, Rh1-shits1 retina, raised at 25°C, with photoreceptor R7 in a healthy state, demonstrating that Rh1-Gal4 overexpresses UAS-shits1 only in photoreceptors R1–R6. Scale = 2 μm. Mitochondria (solid arrows), Multivesicular bodies (open arrows), inter-photoreceptor space (asterisk).