Figure 2.

(A) The NF-κB/DNA complex is still formed in the presence of DEX. TC10 cells were left untreated, or were treated with TNF alone (2,000 units/ml) or with TNF + DEX (10⁻⁶ M) for various periods. The total protein extract was incubated with a ³²P-labeled IL-6-κB response element; protein/DNA complexes were analyzed in an EMSA. Arrowheads indicate the activated κB complex, the constitutively expressed recombination binding protein (RBP)-Jκ and the free probe. (B) DEX treatment does not up-regulate the amount of IκB-α protein. TC10 cells were left untreated or were treated with DEX (10⁻⁶ M) alone, with 2,000 units/ml TNF, or with TNF + 10⁻⁶ M DEX, added at −1 hr. After cell lysis, equal amounts of protein were loaded onto an SDS/polyacrylamide gel for Western blot analysis.