Figure 3.

Repression by DEX acts on κB-driven promoter constructs. L929sA cells with stably integrated p1168hu.IL6P-luc+ (A), p1168hu.IL6P-luc+ NFκBmut (B), p50hu.IL6P-luc+ (C), or p(IL6-κB)₃-50hu.IL6P-luc+ were left untreated (NI), or were treated with DEX alone (10⁻⁶ M) or with TNF (2,000 units/ml) for 6 hr, either alone or with DEX (10⁻⁶ M), added at −1 hr. The relative promoter activity indicates the ratio between the expression levels of the treated and the untreated state, the latter being arbitrarily set to 1. (Inset) Enlargement of the respective induction profiles.