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. 1997 Dec 9;94(25):13504–13509. doi: 10.1073/pnas.94.25.13504

Figure 4.

Figure 4

(A) DEX specifically represses p65-mediated transactivation in L929sA cells. Cells stably transfected with pGal4, pGal4-p65, pGal4-p651–285, pGal4-p65286–551, or pGal4-VP16 were further transiently transfected with 700 ng p(Gal)2-50hu.IL6P-luc+, 700 ng of a plasmid containing β-gal, and carrier DNA, the total being 1.6 μg DNA. After 72 hr, lysates were made and the concentration of luc was determined by using β-gal values as a basis for normalization. In appropriate assays, 10−6 M DEX was added 24 hr before analysis. The normalized luc activity of the untreated samples was arbitrarily set to 100. Error bars show standard deviation of four independent experiments. (B) DEX represses p65 transactivation in TC10 cells. Cells were transiently transfected with pGal4 or pGal4-p65, together with a constant amount of p(Gal)2-50hu.IL6P-luc+, and treated with 10−6 M DEX or untreated. After 24 hr, luc activity was determined by using β-gal values as a basis for normalization. Error bars show standard deviation of four independent transfections.