Figure 5.

DEX does not interfere with Gal4 binding to its DNA motif. L929sA cells containing a stably integrated Gal4-p65 cDNA were left untreated (solid line) or were treated with DEX (10⁻⁶ M) for 12 or 24 hr. Total protein extracts were incubated with ³²P-labeled GAL4 response element for analysis in an EMSA. No complex was observed when a protein extract of parental L929sA cells was incubated with the probe (contr.).