Figure 1.

The enzymatic activity yield on refolding/reoxidation of hen egg-white lysozyme in glycerol containing small fractions of water and in the pure aqueous solvent. Lysozyme was unfolded and reduced in aqueous solution, refolded and reoxidized in one of the glycerol–water mixtures depicted by the shaded bars, and finally assayed in aqueous solution against dried cells of M. lysodeikticus. For experimental conditions, see Materials and Methods and the legend to Table 1. At each glycerol concentration, the refolding yield (i.e., the recovery of the lysozyme activity) was independently measured at least in quadruplicate; the heights of the shaded bars correspond to the mean values, and the error bars correspond to the standard deviations in these experiments.