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Plasmids used for isolation of suppressor mutations in MTF. pRSVCATam1.2.5 (Left) contains the gene for CATam1.2.5 reporter, the mutant tRNA gene, and the gene for E. coli GlnRS. The pACDFMT plasmid (Right) contains the fmt gene for E. coli MTF and was used for the isolation of mutants in the fmt gene by using N-methyl-N′-nitro-N-nitrosoguanidine mutagenesis (details in text). LTR, long terminal repeat.
