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a) Schematic of key characteristics of a F2P1P2 translocation event. Gathering ∼1000 events enables statistically detailed analysis of the mean duration and blockade level of the PNA. Results for both F2P1 (b) and F2P1P2 (c) were nearly identical. Mean blockades for both PNA tags, regardless of number along the DNA, was consistent as ∼0.45 nA and the stalling time of the PNA inside the pore was likewise consistent at ∼200 μs.
