Table 1.

Result Summary (all measurements except exposure time are expressed in millimeters)

Canine Number	Treatment Time	Atrial Septal Axial Motion	ASD			Hemorrhage
			Ultrasound (Center)*	Pathology†		Pathology‡
				RA side	LA side	RA side	LA side
1	6 min	5.2	1.7	3.4	2.9	1.2	1.9
2	10 min	17.1	No ASD was created
3	8 min	8.4	3.3	5.1	2.2	2.3	2.1
4	6 min	10.3	5.0	5.7	2.4	4.2	2.2
5	10 min	10.0	4.2	4.7	2.6	1.6	2.3
6	6 min	8.7	3.0	6.6	3.0	1.6	2.0
7	6 min	9.7	3.0	4.5	2.2	2.3	1.7
8	6 min	6.5	3.2	6.2	2.5	3.2	2.4
9	16 min	6.1	4.0	No pathology result§
10	12 min	8.1	3.6	4.1	3.3	4.0	2.6
mean	8.4 min	8.1	3.4	5.0	2.6	2.6	2.2
std	3.4 min	3.3	0.9	1.1	0.4	1.1	0.3

^*”ASD Ultrasound (Center)” column reports the width of the color jet in the center of the ASD measured on Ultrasound Doppler images.

^†”ASD Pathology RA side” and “LA side” columns show the widths of the acellular zone in the ASD for the right and left atrial surfaces, respectively, measured based on pathology analysis using H&E cross-section slides.

^‡”Hemorrhage Pathology RA side” and “LA side” report the width of the eosinophilic and hemorrhagic rim surrounding the acellular ASD zone for the right and left atrial surfaces, respectively. This width is measured from the outermost edge of the acellular zone to the outermost edge of eosinophilic myocyte and hemorrhage zone on either side of the ASD. For each ASD, this measurement was performed for both sides of the ASD and using multiple slides across the same ASD, and the maximal value of the measurements is reported.

^§Pathological evaluation on the heart of dog #9 could not be obtained because it was filled with heart worms. However, ultrasound Doppler confirmed the ASD creation.