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The evolution (1 5) of the Q band absorption spectrum of a solution phase pH 7.4 met Hb sample (~ 0.45 mM in heme) in the presence of nitrite subsequent to the addition of NO (0.25 mM). After spectrum 5 was obtained (22 minutes post addition of NO), KCN (1 mM) is added (Spectrum a). Spectrum b is the result of subtracting the cyanomet reference spectrum from Spectrum a. Spectrum c is the result of subtracting the initial met nitrite spectrum (Spectrum 1) from Spectrum 5.
