Fig. 3.

Spinal naloxone (NAL) partially blocked the effects of both s.c. [Dmt¹]DALDA ([Dmt1]DAL) and morphine (MOR) in the hot plate test. The hot plate test was performed 2 h ([Dmt¹]DALDA) or 30 min (morphine and saline) after s.c. administration of [Dmt¹]DALDA (0.5 mg/kg), morphine (4.5 mg/kg) or saline (SAL). Intrathecal (i.t.) naloxone (20 μg) or saline were given 10 min prior to testing. The number of animals was 5 or 6 for each group. # Significantly different between groups (p < 0.05). * Significantly different from saline control (p < 0.05).