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. Author manuscript; available in PMC: 2011 Jan 28.
Published in final edited form as: Chem Commun (Camb). 2009 Nov 18;46(4):637–639. doi: 10.1039/b919815f

Fig. 4.

Fig. 4

Activation of PEST-CC-204 in a complex proteome. Crude cell lysates from E. coli strains expressing either WT PEST or PEST-CC-204 were incubated in the absence or presence (10 µM) of FlAsH. After 2.5 hours, the PTP activities of the lysates were assayed as in Figure 1. To normalize the data from different lysates, the measured maximum velocity (µM s−1) of each lysate divided by its respective total protein concentration (mg mL−1). Error bars represent the standard deviations from two (WT) and four (PEST-CC-204) independent lysis experiments.