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. Author manuscript; available in PMC: 2010 Mar 8.
Published in final edited form as: J Immunol. 2008 Mar 15;180(6):4218–4226. doi: 10.4049/jimmunol.180.6.4218

FIGURE 2.

FIGURE 2

LPS activation of Akt and MAPK pathways in WT and Pik3r1−/− PECs. WT and Pik3r1−/− PECs were stimulated with LPS (1 μg/ml) for 0 –20 min. The activation of Akt (A), ERK1/2 (B), JNK1/2 (C), and p38 (D) was determined in whole cell protein extracts by Western blotting using anti-phospho specific Abs. Each blot was stripped and reprobed for the non-phosphorylated form of each protein to assess loading. Representative western blots are shown. Normalized levels of phosphorylated proteins are shown as mean ± SEM (n = 3– 6) relative to WT cells stimulated with LPS for 20 min (defined as 100%).*, p < 0.05.