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. Author manuscript; available in PMC: 2010 Mar 8.
Published in final edited form as: J Immunol. 2008 Mar 15;180(6):4218–4226. doi: 10.4049/jimmunol.180.6.4218

FIGURE 4.

FIGURE 4

LPS induction of Egr-1 in WT and PTEN−/− PMs, and WT and Pik3r1−/− PECs. WT and PTEN−/− PMs (A–C) or WT and Pik3r1−/− PECs (D–F) were stimulated with LPS (1 μg/ml) and the induction of Egr-1 mRNA was determined (A and D). Levels of Egr-1 and Sp1 in nuclear extracts of PMs stimulated for 120 min were determined by EMSA. Sp1 was used to assess loading. Representative EMSAs are shown. Egr-1 mRNA levels normalized to levels of GAPDH are shown as mean ± SEM (n = 5– 6) relative to WT cells stimulated with LPS (defined as 100%). Egr-1 levels normalized to levels of Sp1 are shown as mean ± SEM (n = 3– 6) relative to WT cells stimulated with LPS (defined as 100%). *, p <0.05.