Effect of Pik3r1 or p85α deficiency on inflammation and coagulation in endotoxemic mice. A, Genotyping of blood cells from WT mice containing either WT or Pik3r1−/− bone marrow (4 mice per group). B, p85α protein levels in blood cells isolated from WT mice containing either WT or Pik3r1−/− bone marrow. WT mice with either WT or Pik3r1−/− bone marrow were injected with LPS (5 mg/kg) and the plasma levels of TNF-α (C), IL-6 (D), and TAT (E) (8 h) were determined. n = 20 –30 mice for each group. WT and p85α−/− mice were injected with LPS (5 mg/kg) and the plasma levels of TNF-α (F), IL-6 (G), and TAT (H) (8 h) were determined. n = 10 mice for each group. Data are expressed as mean ± SEM.*, p < 0.05.