Figure 4.

Visualization of infected OBP adenoviruses by green fluorescent protein (GFP) expression. To visualize OBP-301 in vitro and in vivo, the GFP gene driven by the cytomegalovirus (CMV) promoter was inserted into the genome of OBP-301, named OBP-401 (a). SKOV3 and normal human fibroblasts were infected with OBP-401 at an multiplicity of infection (MOI) of 10 and GFP expression was assessed using a fluorescence microscope (b). SKOV3 cells (1 × 10⁷ cells) were inoculated into the peritoneal cavity of BALB/c nu/nu mice and OBP-401 was injected into the peritoneal cavity 4 weeks later. The mice were killed and GFP expression was assessed 72 h after injection of OBP-401 (c).