Figure 2.

Bioelectrochemical reactor for the electrode-driven P450cam biocatalytic cycle. The reactor is modified from a 40-ml cylindrical glass vessel (6 cm high). Two tin oxide working electrodes with a total surface area of 16 cm² were wrapped in Pt mesh (not shown) that had no electric contact and was at open circuit potential during electrolysis. At the point of minimum separation, the working electrodes were about 1 mm from the Pt grid counter electrode. The extent of Pt grid counter electrode submergence was varied to maximize electrochemical oxygen evolution without causing chlorine evolution, which is severely detrimental to activity. Details concerning operation of the reactor during electrolysis are given in the text.