Table 1.
Reactor solution composition* | Oxygen condition | Product turnover rate, nmol of 5-exo-camphor/min per nmol of CYP101 |
---|---|---|
41 μM Pdx | Ar purge | 0 |
12.8 μM CYP101 | Ar purge | 0 |
41 μM Pdx + 0.41 μM CYP101 | Air saturated | 0.9 |
55 μM Pdx + 0.18 μM CYP101 | Ar purge | 4.2 |
108 μM Pdx + 0.23 μM CYP101 | Ar purge | 6.4 |
1030 μM Pdx + 0.13 μM CYP101† | Ar purge | 34 |
1030 μM Pdx + 0.13 μM CYP101‡ | Ar purge | 36 |
Standard experimental electrolysis reaction conditions: buffer was 50 mM Tris⋅HCl, 0.18 M KCl. 16 mM MgCl2; 800 μM initial camphor concentration; Ewe = −0.7 V on the tin oxide working electrode; temperature was 21–22°C.
Standard condition except initial camphor concentration was 2 mM.
Standard condition except initial camphor concentration was 2 mM and temperature was 37°C.