Skip to main content
. Author manuscript; available in PMC: 2010 Jul 28.
Published in final edited form as: Nature. 2010 Jan 6;463(7280):554–558. doi: 10.1038/nature08732

Figure 2. Knockdown of Elp3 impairs DNA demethylation in the paternal pronucleus.

Figure 2

(a) siRNA-mediated knockdown of Elp3 resulted in increased 5mC staining (green) in the PN5 paternal pronucleus. H3.3-mRFP1 (red) serves as a nuclear marker. H3.3-mRFP1 signal is more intense in male pronuclei than in female pronulcei due to preferential incoporation of H3.3 to the paternal genome. ♂: male pronucleus, ♀: female pronucleus, PB: polar body. Bar = 25 μm. (b) Quantification of the ratio (male/female) of 5mC intensity in Elp3 knockdown and control groups. Each dot represents a zygote. Red bars represent the average ratio of each group. The statistics of the injections are presented in the table. (c) Bisulfite sequencing of Line1-5′ and ETn indicates that knockdown of Elp3 impairs paternal DNA demethylation. Open circles and closed circles represent unmethylated and methylated CpG respectively. Each line represents an individual clone. 10 CpGs and 15 CpGs were analyzed for Line1-5′ and ETn respectively.